Homepage of the Lentiviral Gene Ontology Vectors

The links on this page lead to external web pages. I don't have any influence on these web pages.

{List of used fluorescent proteins}
Here you can find a very informative review about fluorescent proteins in general:
A guide to choosing fluorescent proteins (PubMed)

Name Excitation Emission Color External Links
Cerulean 433nm 475nm   PubMed1, PubMed2
eGFP 484nm 507nm   PubMed1, PubMed2
Emerald (EmGFP) 487nm 509nm   PubMed1, PubMed2, PubMed3
eYFP 514nm 527nm   PubMed
Venus 515nm 528nm   PubMed1, PubMed2
dsRed2 563nm 582nm   Clontech
dsRedExpress 555nm 584nm   Clontech
tdTomato (tandem dimer) 554nm 581nm   PubMed1, PubMed2
dTomato 554nm 581nm   PubMed1, PubMed2
mCherry 587nm 610nm   PubMed1, PubMed2

Keep in mind that in Cytometry you may have suboptimal excitation of the fluorescent proteins by the fixed laser wavelengths of the machine. In microscopy it is easier to get adapted filter sets that could help to increase brightness. And Cerulean is not visible using DAPI filters in microscopy.

{Fluorescence resistance fusion proteins}

For all fusion proteins cloned so far, the spectral properties appear to be unchanged (excitation and emission wavelengths). But the fusion proteins are always less bright, maybe because of transcription/translation efficiencies or mRNA/protein stability. It has not been determined if the spectral properties of the fused proteins are really identical to the properties of the fluorescent proteins alone.

  © 2015 · Kristoffer Riecken · contact